Chitosan and L-histidine coated biofunctional TiO? composite with enhanced ROS-mediated antimicrobial and anticancer efficacy for biomedical applications
- Title
- Chitosan and L-histidine coated biofunctional TiO? composite with enhanced ROS-mediated antimicrobial and anticancer efficacy for biomedical applications
- Creator
- Thangavelu, Indumathi; Tadepalli, Srinivas; A. AlGhamdi, AbdulAziz; Boopathi, Thalakulam Shanmugam
- Description
- Societies continue to face the urgent challenge of developing effective, safe, and stable nanoparticles with promising biomedical applications. Herein, the present study synthesis chitosan and L-Histidine dually coated titanium dioxide (TiCSLH) nanocomposite (NC) by chemical co-precipitation method. The antimicrobial performance of TiO? nanoparticles is closely linked to their physicochemical properties, such as reduced particle size, increased bandgap, and the presence of oxygen vacancies, all of which collectively enhance reactive oxygen species (ROS) generation and inhibit microbial growth. Structural analyses using XRD and TEM confirmed that TiCSLH nanoparticles possess an anatase phase and a spherical morphology. Compared to TiO?, TiCSLH nanoparticles demonstrated significantly enhanced antimicrobial activity against MRSA, E. coli, K. pneumoniae, P. aeruginosa and C. albicans strains, as evidenced by larger zones of inhibition from 2324mm. This enhanced efficacy is attributed to their smaller particle size (~ 26 3nm), wider bandgap (3.34eV), and prominent oxygen vacancy-related emissions at 518 and 531nm, which facilitate increased ROS production, resulting in cellular membrane disruption and microbial death. Furthermore, compared to TiO2, TiCSLH exhibited notable anticancer potential against breast cancer cells, with an IC?? of 10?g/mL, while maintaining excellent biocompatibility, as evidenced by its non-toxic response toward L929 fibroblast cells, which showed 83.5% cell viability. Collectively, these findings underscore the potential of TiCSLH nanoparticles for diverse biomedical applications. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2025.
- Source
- Naunyn-Schmiedeberg's Archives of Pharmacology;Volume;398;Issue;12;pp.17895-17906
- Date
- 01-01-2025
- Publisher
- Springer Science and Business Media Deutschland GmbH
- Subject
- Breast cancer cell; Chitosan; L-Histidine; MRSA; TiO2
- Coverage
- Thangavelu I., Department of Chemistry, CHRIST (Deemed to Be University), Karnataka, Bangalore, 560029, India; Tadepalli S., Department of Chemical Engineering, College of Engineering, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, 11432, Saudi Arabia; A. AlGhamdi A., Department of Chemical Engineering, College of Engineering, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, 11432, Saudi Arabia; Boopathi T.S., Department of Chemistry, Amrita School of Physical Sciences Coimbatore, Amrita Vishwa Vidyapeetham, Coimbatore, 641112, India, Functional Materials Laboratory, Amrita School of Engineering Coimbatore, Amrita Vishwa Vidyapeetham, Coimbatore, 641112, India
- Rights
- Restricted Access; Hardcopy may be available in the library
- Relation
- ISSN: 281298; CODEN: NSAPC
- Format
- online
- Language
- English
- Type
- Article
Collection
Citation
Thangavelu, Indumathi; Tadepalli, Srinivas; A. AlGhamdi, AbdulAziz; Boopathi, Thalakulam Shanmugam, “Chitosan and L-histidine coated biofunctional TiO? composite with enhanced ROS-mediated antimicrobial and anticancer efficacy for biomedical applications,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 19, 2026, https://archives.christuniversity.in/items/show/21831.