ItemArticle

Fabrication of Kaempferol Loaded Zein Nanoparticles: Investigation of in Vitro Cytotoxicity and Apoptosis Induction in Oral Cancer Cells

Title
Fabrication of Kaempferol Loaded Zein Nanoparticles: Investigation of in Vitro Cytotoxicity and Apoptosis Induction in Oral Cancer Cells
Creator
Roopashree, R.; Gupta, Anchal; Rathore, Mahendra Singh; Sharma, Kamini; Chinnathambi, Arunachalam; Alharbi, Sulaiman Ali; Ramar, Mohankumar; Kalaiarasi, Giriraj; Thangavelu, Indumathi; Suriyaprakash, Jagadeesh
Description
Oral cancer remains a significant health challenge, necessitating innovative therapeutic strategies to enhance treatment efficacy and minimize side effects. This study investigates the potential of kaempferol-loaded zein nanoparticles (KZNPs) for this purpose. Kaempferol, a flavonoid with anticancer properties, has poor water solubility, limiting its effectiveness. Zein nanoparticles (ZNPs) offer a promising delivery system for such bioactive compounds. UV-Vis spectroscopy identified Kaempferols absorption peaks at 347 and 253nm, which shifted to 338nm when encapsulated in ZNPs, indicating a change in ??* conjugation. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) confirmed that sodium caseinate (SC) stabilizes ZNPs, resulting in spherical particles with optimal size and stability. Fourier transform infrared (FTIR) spectroscopy suggested enhanced hydrogen bonding between Kaempferol and zein. Differential scanning calorimetry (DSC) revealed the absence of Kaempferols crystalline peaks in KZNPs. The encapsulation efficiency (EE) was 98.39%, and drug release studies showed a controlled release of 79% kaempferol over 8h. In vitro assays demonstrated that KZNPs significantly increased Kaempferols cytotoxicity against PCI-13 oral cancer cells without affecting normal NIH3T3 cancer cells. Overall, these results demonstrate that our KZNPs enhanced biocompatibility and anticancer properties for oral cancer cells. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025.
Source
Journal of Polymers and the Environment;Volume;33;Issue;5;pp.2371-2384
Date
01-01-2025
Publisher
Springer
Subject
Apoptosis; Cytotoxicity; Kaempferol; Oral cancer; Zein
Coverage
Roopashree R., Department of Chemistry and Biochemistry, JAIN (Deemed-to-be University), Karnataka, Bangalore, 560069, India; Gupta A., Centre of Research Impact and Outcome, Chitkara University, Punjab, Rajpura, 140417, India; Rathore M.S., Department of Applied Science, PIT, Parul University, Gujarat, Vadodara, 391760, India; Sharma K., Department of Sciences, Vivekananda Global University, Jaipur, 303012, India; Chinnathambi A., Department of Botany and Microbiology, College of Science, King Saud University, PO Box - 2455, Riyadh, 11451, Saudi Arabia; Alharbi S.A., Department of Botany and Microbiology, College of Science, King Saud University, PO Box - 2455, Riyadh, 11451, Saudi Arabia; Ramar M., Department of Pharmacology and Toxicology, School of Pharmacy, University of Connecticut, Storrs, CT, United States; Kalaiarasi G., Centre for Material Chemistry, Department of Chemistry, Karpagam Academy of Higher Education, Tamil Nadu, Coimbatore, 641 021, India; Thangavelu I., Department of Chemistry, CHRIST (Deemed to be University), Karnataka, Bangalore, 560029, India; Suriyaprakash J., Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510006, China
Rights
Restricted Access; Hardcopy may be available in the library
Relation
ISSN: 15662543; CODEN: JPENF
Format
online
Language
English
Type
Article

Citation

Roopashree, R.; Gupta, Anchal; Rathore, Mahendra Singh; Sharma, Kamini; Chinnathambi, Arunachalam; Alharbi, Sulaiman Ali; Ramar, Mohankumar; Kalaiarasi, Giriraj; Thangavelu, Indumathi; Suriyaprakash, Jagadeesh, “Fabrication of Kaempferol Loaded Zein Nanoparticles: Investigation of in Vitro Cytotoxicity and Apoptosis Induction in Oral Cancer Cells,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 19, 2026, https://archives.christuniversity.in/items/show/21919.

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