ItemArticle

Stepwise hydrothermally synthesized gold nanoparticles supported copper metal-organic frameworks as an impedimetric immunosensor for the ultrasensitive detection of pancreatic cancer

Title
Stepwise hydrothermally synthesized gold nanoparticles supported copper metal-organic frameworks as an impedimetric immunosensor for the ultrasensitive detection of pancreatic cancer
Creator
Chen, Xiao-Qi; Vijayaraghavan, Priya; Palanisamy, Sathyadevi; Amer, Tarik Abdelkareem Mostafa; Lu, Tsai-Te; Chen, Chiao-Yun; Wang, Yun-Ming
Description
Carbohydrate antigen (CA199) is a frequently used biomarker for detecting and prognosis of pancreatic cancer. Early detection of pancreatic cancer remains a challenge in routine clinical analyses, including imaging techniques such as magnetic resonance imaging, ultrasonography, and computed tomography. There is an urgent urge to develop robust sensors like electrochemical immunosensors that provide low-priced and sensitive biomarker detection. A potential electrochemical immunosensor comprising Au nanoparticles supported on Cu MOF, HKUST-1 (Au@HKUST-1) on screen-printed carbon electrodes (SPCE) was developed via a one-pot stepwise hydrothermal method for the ultralow level detection of CA 199 in human serum using electrochemical impedance spectroscopy (EIS). CA 199 could be detected in a wide range of concentrations, including 0.01 U mL-1 to 35 U mL-1. Au@HKUST-1/SPCE sensor displayed ultralow levels of detection of CA 199 with a limit of detection (LOD) in PBS as 0.17 U mL-1 and serum as 0.08 U mL-1. The limit of quantification of the immunosensor is 0.53 U mL-1 and 0.26 U mL-1 in PBS and serum, respectively. This work also emphasizes the ultrasensitive detection of pancreatic cancer using CA 199 biomarker in serum samples, and achieved a reliable analytical platform for the detection of CA 199 and other biomarkers. 2025 Elsevier Ltd.
Source
Electrochimica Acta;Volume;542;Issue;;Article No.;147524;
Date
01-01-2025
Publisher
Elsevier Ltd
Subject
Au nanoparticles; Electrochemical sensor; Human serum; Metal-organic frameworks; Pancreatic cancer
Coverage
Chen X.-Q., Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan; Vijayaraghavan P., Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan, Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, 300, Taiwan, Department of Chemistry, Christ University, Hosur Road, Bengaluru, 560029, India; Palanisamy S., Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan, Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, 300, Taiwan; Amer T.A.M., Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan; Lu T.-T., Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, 300, Taiwan, Department of Chemistry, National Tsing Hua University, Hsinchu, 30013, Taiwan, Department of Chemistry, Chung Yuan Christian University, Taoyuan, 32023, Taiwan; Chen C.-Y., Department of Medical Imaging, Kaohsiung Medical University Hospital, Kaohsiung, 807, Taiwan, School of Post-Baccalaureate Medicine, College of Medicine, Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung, 807, Taiwan; Wang Y.-M., Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, Center for Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan, Department of Biomedical Science and Environmental Biology, School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, 807, Taiwan
Rights
Restricted Access; Hardcopy may be available in the library
Relation
ISSN: 134686; CODEN: ELCAA
Format
online
Language
English
Type
Article

Citation

Chen, Xiao-Qi; Vijayaraghavan, Priya; Palanisamy, Sathyadevi; Amer, Tarik Abdelkareem Mostafa; Lu, Tsai-Te; Chen, Chiao-Yun; Wang, Yun-Ming, “Stepwise hydrothermally synthesized gold nanoparticles supported copper metal-organic frameworks as an impedimetric immunosensor for the ultrasensitive detection of pancreatic cancer,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 20, 2026, https://archives.christuniversity.in/items/show/22242.

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