ItemArticle

Development of a natural product-based selective fluorescent sensor for Cu2+ and DNA/protein: insights from docking, DFT, cellular imaging and anticancer activity

Title
Development of a natural product-based selective fluorescent sensor for Cu2+ and DNA/protein: insights from docking, DFT, cellular imaging and anticancer activity
Creator
Pakrashy, Sourav; Das, Manik; Manna, Sounik; Choudhury, Sujata Maiti; Shinziya, Hazeena; Das, Bhriguram; Dolai, Malay; Das, Avijit Kumar
Description
The natural product seselin (SS), was synthesized and characterized spectroscopically for the selective detection of Cu2+ and biomolecules such as ct-DNA and BSA. The probe exhibits strong bluish emission in a MeOH-H2O (7 : 3, v/v) HEPES buffer solution (pH 7.4) at 453 nm. Upon exposure to Cu2+, the SS solution shows a selective fluorescence turn-off with a binding constant of 2.13 105 M?1 and a detection limit of 3.48 10?8 M. The HOMO-LUMO energy gap of the probe SS decreases from ?E = 7.97 eV to ?E = 7.77 eV upon binding with Cu2+, indicating enhanced stability due to ligand-metal complex formation. Significantly, the ligand SS exhibits fluorescence enhancement in the presence of ct-DNA and BSA, resulting in a visible fluorescence change from colorless to blue, with binding constants of 4.8 104 M?1 and 4.7 104 M?1, respectively. The binding interactions of SS with biomacromolecules have been explored through molecular docking studies, revealing that the probe can serve as a promising anti-cancer and anti-viral agent. Furthermore, the probe SS demonstrates potent anticancer activity in treatments involving MCF-7 and HLC cells. Additionally, the probe SS is capable of detecting intracellular Cu2+ in live MCF-7 cell lines. 2025 The Royal Society of Chemistry.
Source
Analytical Methods;Volume;17;Issue;30;pp.6160-6169
Date
01-01-2025
Publisher
Royal Society of Chemistry
Coverage
Pakrashy S., Department of Chemistry, Prabhat Kumar College, WB, Purba Medinipur, 721404, India; Das M., Department of Chemistry, Prabhat Kumar College, WB, Purba Medinipur, 721404, India; Manna S., Biochemistry, Molecular Endocrinology and Reproductive Physiology Laboratory, Department of Human Physiology, Vidyasagar University, WB, Midnapore, 721102, India; Choudhury S.M., Biochemistry, Molecular Endocrinology and Reproductive Physiology Laboratory, Department of Human Physiology, Vidyasagar University, WB, Midnapore, 721102, India; Shinziya H., Department of Chemistry, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; Das B., Department of Chemistry, Vidyasagar University, WB, Paschim Medinipur, 721102, India; Dolai M., Department of Chemistry, Prabhat Kumar College, WB, Purba Medinipur, 721404, India; Das A.K., Department of Chemistry, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India
Rights
All Open Access; Hybrid Gold Open Access
Relation
ISSN: 17599660;
Format
online
Language
English
Type
Article

Citation

Pakrashy, Sourav; Das, Manik; Manna, Sounik; Choudhury, Sujata Maiti; Shinziya, Hazeena; Das, Bhriguram; Dolai, Malay; Das, Avijit Kumar, “Development of a natural product-based selective fluorescent sensor for Cu2+ and DNA/protein: insights from docking, DFT, cellular imaging and anticancer activity,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 18, 2026, https://archives.christuniversity.in/items/show/22551.

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