ItemArticle

Exploring a GE/Nafion/Co-MOF nanosheets/CuO NPs/GOx powered electrochemical biosensor for ultrasensitive detection of rebaudioside A

Title
Exploring a GE/Nafion/Co-MOF nanosheets/CuO NPs/GOx powered electrochemical biosensor for ultrasensitive detection of rebaudioside A
Creator
Manuel, Manju; Kanchi, Suvardhan; Losetty, Venkatramana
Description
Rebaudioside A (Reb A) is a natural, non-nutritive sweetener highly prevalent in the global sweetener market and widely preferred by consumers. In this study, an advanced electrochemical biosensor was developed for sensing Reb A, using a modified graphite rod electrode extracted from discharged ZnC batteries. The electrode was fabricated using a layer-by-layer strategy with Nafion, Co-MOF nanosheets, CuO NPs, and glucose oxidase (GOx) enzyme. The nanomaterials were characterized by UV-vis, FTIR, DLS, zeta potential measurements, XRD, Raman, SEM, TEM, EDS, and XPS techniques. Electrochemical characterization via Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) revealed a significant enhancement in electrical conductivity and increased electroactive surface area. The designed biosensor exhibited a sharp oxidation peak at 0.16 V due to ester bond cleavage in Reb A, which was further amplified in the presence of GOx, resulting from hydroxyl oxidation and hydrogen peroxide generation. Differential pulse voltammetry (DPV) demonstrated a linear response over a concentration range of 2.014 M (R2 = 0.993) with a limit of detection (LOD) of 0.23 M. The sensor displayed excellent analytical performance, with repeatability, reproducibility (RSD = 3.9%), and stability. Additionally, recovery studies confirmed its accuracy, ranging from 97% to 98.17%. Further, the molecular docking studies confirmed strong Reb AGOx interactions (?7.26 kcal mol?1), supporting the biosensor's specificity. The developed biosensor demonstrates excellent analytical performance, making it highly suitable for routine laboratory analysis of sweeteners in complex food matrices. This journal is The Royal Society of Chemistry, 2026
Source
Nanoscale Advances;Volume;8;Issue;1;pp.167-180
Date
01-01-2026
Publisher
Royal Society of Chemistry
Coverage
Manuel M., Department of Chemistry, CHRIST University, Bengaluru, 560 029, India, Centre for Renewable Energy and Environmental Sustainability, CHRIST University, Bengaluru, 560 029, India; Kanchi S., Department of Chemistry, CHRIST University, Bengaluru, 560 029, India, Centre for Renewable Energy and Environmental Sustainability, CHRIST University, Bengaluru, 560 029, India; Losetty V., Department of Chemistry, Vel Tech Rangarajan Dr Sagunthala R&D Institute of Science and Technology, Avadi, Chennai, 600 062, India
Rights
All Open Access; Gold Open Access; Green Open Access
Relation
ISSN: 25160230;
Format
online
Language
English
Type
Article

Citation

Manuel, Manju; Kanchi, Suvardhan; Losetty, Venkatramana, “Exploring a GE/Nafion/Co-MOF nanosheets/CuO NPs/GOx powered electrochemical biosensor for ultrasensitive detection of rebaudioside A,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 18, 2026, https://archives.christuniversity.in/items/show/22563.

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