ItemArticle

Effect of heavy metal elicitation on antioxidants and andrographolide content in cell suspension cultures of Andrographis paniculata

Title
Effect of heavy metal elicitation on antioxidants and andrographolide content in cell suspension cultures of Andrographis paniculata
Creator
Ahmed, Nihal; Nagella, Praveen
Description
Andrographolide, a bicyclic diterpene from Andrographis paniculata is of immense pharmaceutical importance. A. paniculata, an annual herb from Acanthaceae is widespread in the Indian subcontinent. Heavy metals act as abiotic elicitors. The study deals with the effect of mercury (Hg), cadmium (Cd) and arsenic (As) on andrographolide content, phenols and flavonoids and each of their correlation with the metal chelating and radical scavenging activity, in cell suspension cultures of A. paniculata. Andrographolide was estimated using HPLC, while other estimation methods were used for other metabolites. Four different concentrations of each of the heavy metal salts CdCl2, As2O3and HgCl2, were administered in liquid MS media containing 1 g of cells. Media without any metal served as control. Higher concentrations of Cd and As imparted a positive effect on andrographolide content, Hg imparted a negative effect. The cells were most sensitive to Hg and most tolerant to Cd. Cd could be the best choice as an elicitor for increased production of andrographolide. While phenols show a positive correlation with antioxidants, flavonoids and andrographolides do not show a positive correlation with antioxidants. The Author(s).
Source
Plant Science Today;Volume;12;Issue;2;
Date
01-01-2025
Publisher
Horizon e-Publishing Group
Subject
Andrographis paniculata; andrographolide; arsenic; cadmium; flavonoids; mercury; phenols
Coverage
Ahmed N., Department of Life Science, CHRIST (Deemed to be University), Bengaluru, 560 029, India; Nagella P., Department of Life Science, CHRIST (Deemed to be University), Bengaluru, 560 029, India
Rights
All Open Access; Gold Open Access; Green Open Access
Relation
ISSN: 23481900;
Format
online
Language
English
Type
Article

Citation

Ahmed, Nihal; Nagella, Praveen, “Effect of heavy metal elicitation on antioxidants and andrographolide content in cell suspension cultures of Andrographis paniculata,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 18, 2026, https://archives.christuniversity.in/items/show/23272.

Output Formats