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Bioconversion of Feather Composts using Proteolytic Bacillus mycoides for their Possible Application as Biofertilizer in Agriculture
Proteolytic Bacillus strains were screened for highest protease production amongst which Bacillus mycoides (G2) was chosen as an assuring protease producer. Enzyme activity was maximum at 37C, pH-7, when the medium was supplemented with 0.5 and 0.75% of sucrose and beef extract respectively. Tapioca flour and soybean meal were capable of replacing commercial carbon and nitrogen sources respectively. Feather degradation studies revealed 62% of degradation with Quail feather (QF), followed by Chicken feather (CF) (58%), Guinea fowl feather (51%) and Pigeon feather (43%). Biodegradation of feather samples in soil evidenced degradation of Quail feather and Chicken feather at the following patternQF Treatment 1 (5%) ? CF Treatment 1 (5%) ? QF Treatment 2 (10%) ? CF Treatment 2 (10%). Maximum degradation of QF and sufficient release of free amino acids into the feather compost was obvious with Field Emission Scanning Electron Microscopic (FE-SEM) and High Performance Thin Layer Chromatographic (HPTLC) analyses respectively. In vitro plant growth studies of tomato and chilly plants were accomplished with feather composts. Maximum growth of 26.44cm (shoot length) was achieved when feather compost prepared with degraded QF (5%) was utilized as plant growth substrate, than other treatment pots (P < 0.05). Plant growth was exemplary in the case of tomato when compared to that of chilly. Sound degradation of QF, followed by CF using Bacillus mycoides could strengthen the efficacy of microbial fermentation processes. This significant attempt could support poultry farms as well as organic agricultural sectors ecologically. Graphic Abstract: [Figure not available: see fulltext.] 2021, The Author(s), under exclusive licence to Springer Nature B.V. -
Biodegradation of polypropylene films by Bacillus paralicheniformis and Lysinibacillus fusiformis isolated from municipality solid waste contaminated soil
The fossil fuel or petroleum derived plastics are applied in our routine life because of their easy availability. Distribution and contamination of the plastics in the landfills are the major reasons for these biodegradation study. This current study reveals the biodegradation of polypropylene films and the growth of Bacillus paralicheniformis and Lysinibacillus fusiformis isolated from plastic contaminated soil collected from municipality solid waste management site. The degradation rate of PP films was confirmed by the results of biodegradation analysis. The growth of Bacillus paralicheniformis and Lysinibacillus fusiformis had shown OD values at 600nm after the degradation period of 4 weeks increasing from 0.131 to 0.334 and 0.148 to 0.213 respectively. The viable cell count increased from 804cells/ml to 1204cells/ml and 10.104cells/ml to 15.204cells/ml respectively. The physical and chemical changes of PP films were confirmed by FT-IR and XRD analysis. These analysis confirmed that the bacterial strains have the ability to change the chemical and physical nature of PP films and can utilize the PP films as sole carbon source. 2021 World Research Association. All rights reserved. -
Biodegradation studies of polyhydroxyalkanoates extracted from Bacillus subtilis NCDC 0671
The major characteristic feature that distinguishes polyhydroxyalkanoates (PHAs) from its synthetic counterparts is its biodegradability. PHAs are the only class of biopolymers reported to be 100% degradable under both aerobic and anaerobic conditions without production of any toxic residues. The biodegradability of PHAs is influenced by several factors like moisture, temperature, pH, surface area and molecular weight of the polymer. The rate of biodegradation varies greatly depending on the environment. Biodegradation studies were carried out using plating method and direct inoculation method using selected Bacillus strains. Fungal degradation of PHA sheets was assessed using Penicillium chrysogenum. Biodegradation of PHA sheets in different soil types like river valley, agricultural land and garden soil was investigated. The degree of PHA degradation in aqueous environment was studied by incubating the sheets in distilled water, sea water, fish tank water and pond water. The highest degradation rate was observed with agriculture land soil (35.47 0.13%) and fish tank soil (36.93 0.13%). The non-toxic nature of the soil incubated with PHA sheets was ensured using plant growth test. 2019, World Research Association. All rights reserved. -
Biodegradation studies of polyhydroxyalkanoates extracted from bacillus subtilis NCDC 0671 /
Research Journal of Chemistry And Environment, Vol.23, Issue 6, pp.107-114 -
Bioengineering of biowaste to recover bioproducts and bioenergy: A circular economy approach towards sustainable zero-waste environment
The inevitable need for waste valorisation and management has revolutionized the way in which the waste is visualised as a potential biorefinery for various product development rather than offensive trash. Biowaste has emerged as a potential feedstock to produce several value-added products. Bioenergy generation is one of the potential applications originating from the valorisation of biowaste. Bioenergy production requires analysis and optimization of various parameters such as biowaste composition and conversion potential to develop innovative and sustainable technologies for most effective utilization of biowaste with enhanced bioenergy production. In this context, feedstocks, such as food, agriculture, beverage, and municipal solid waste act as promising resources to produce renewable energy. Similarly, the concept of microbial fuel cells employing biowaste has clearly gained research focus in the past few decades. Despite of these potential benefits, the area of bioenergy generation still is in infancy and requires more interdisciplinary research to be sustainable alternatives. This review is aimed at analysing the bioconversion potential of biowaste to renewable energy. The possibility of valorising underutilized biowaste substrates is elaborately presented. In addition, the application and efficiency of microbial fuel cells in utilizing biowaste are described in detail taking into consideration of its great scope. Furthermore, the review addresses the significance bioreactor development for energy production along with major challenges and future prospects in bioenergy production. Based on this review it can be concluded that bioenergy production utilizing biowaste can clearly open new avenues in the field of waste valorisation and energy research. Systematic and strategic developments considering the techno economic feasibilities of this excellent energy generation process will make them a true sustainable alternative for conventional energy sources. 2023 Elsevier Ltd -
Biofuel production and characterization from waste chicken skin and pig fat
The biofuels are the most important alternative energy sources in future to fulfil the energy demands. The team of our students carried out an innovative process to convert waste to value-added products. The students have been visited many meat stalls and gathered the required amount of resources with and without cost. The collected waste chicken skin and pig tallow is heated and extracted fat, which is the primary sources to produce the biofuel. The fat extraction process was carried by shredding down the waste chicken skin and pig tallow. The obtained fat was filtered and heated up to 110C to remove all the impurities, water suspensions, blood cells and pieces of bones. The process called transesterification process was carried out to convert obtained fat into biofuel with methyl alcohol and KOH as a catalyst. Transesterification process carted with fat before acid wash and after acid wash to examine the effect of FFA on biofuel yield. The quantity of biofuel yield has been observed to be 62 to 68% for fat from waste chicken skin and 82 to 83 % for fat from pig tallow. The derived fuel from fat from both resources is combined with conventional diesel fuel to check the different properties on a volume basis varied by 10% up to 40%. The essential properties such as viscosity, density, flashpoint, fire point and calorific values were determined, and results show that the fuel combination CB20 and PB20 meets the all requirements of ASTM standards to fix as an additive fuel to CI engines. The clear biofuel from both the fat expressed higher viscosity, density, flash and fire point with a lesser value of energy density. BEIESP. -
Biofuel production and characterization from waste chicken skin and pig fat /
International Journal of Recent Technology And Engineering, Vol.8, Issue 3, pp.3598-3603, ISSN No: 2277-3878. -
Biogenesis and Green Synthesis of Metal Nanoparticles and Their Pharmacological Applications
Nanomaterial innovation is the primary catalyst of advancement in nanotechnology. Although there are many known chemical processes for creating nanoparticles that use harmful substances, it is now more important than ever to use processes that are safer, greener, and more environmentally friendly. The goal of research in this field is to use diverse life forms as "nanoparticle factories." Phytochemicals can convert salt into the appropriate nanoparticles thanks to their regular biosynthetic routes. In recent years, green chemistry methods for the synthesis of metallic nanoparticles have emerged as a fresh and exciting area of study. Metal nanoparticles, including gold (Au), silver (Ag), iron (Fe), and cadmium (Cd) along with certain oxides, can be synthesized using a variety of chemical and physical techniques as well as biological techniques carried out using plants. It has been discovered that methods involving plant-mediated synthesis are a more efficient and cost-effective way to create these metal nanoparticles. The plant-mediated nanoparticles are used as potential pharmaceutical agents for many diseases, including hepatitis, cancer, malaria, and HIV. Due to the higher efficacy and fewer side effects of nanodrugs compared to other commercial cancer drugs, the synthesis of nanoparticles targeting biological pathways has gained tremendous popularity. This review paper aims to cover the different green methods for the biogenesis of these nanoparticles, the different compounds and salts used, and the metals obtained. Ultimately, the significance and prospects of these metal nanoparticles especially in the fields of medicine, pharmacology, drug designing, and drug delivery engineering will also be commented on. The Author(s). -
Biogenic synthesis of dopamine/carboxymethyl cellulose/TiO2 nanoparticles using Psidium guajavaleaf extract with enhanced antimicrobial and anticancer activities
The green synthesis of metal oxide nanoparticles (NPs) has garnered considerable attention from researchers due to its utilization of eco-friendly solvents during synthesis and cost-effective approaches. This study focuses on the synthesis of titanium oxide (TiO2) and dopamine (DA) carboxymethyl cellulose (CMC)-doped TiO2 (DA/CMC/TiO2) NP using Psidium guajava leaf extract, while also investigating the structural, optical, and morphological and biocidal potential of the prepared NPs. Significantly larger zones of inhibition were observed for DA/CMC/TiO2 NPs compared to TiO2 against various pathogens. Moreover, the MTT assay was carried out to evaluate the anticancer activity of the prepared samples against MG-63 cells, and the results revealed that DA/CMC/TiO2 NPs exhibited significantly higher level of anticancer activity compared to TiO2. The experimental results demonstrated that DA/CMC/TiO2 NPs exhibited enhanced anticancer activity in a dose-dependent manner when compared to TiO2 NPs. 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. -
Biogenic synthesis of g-C3N4/Bi2O3 heterojunction with enhanced photocatalytic activity and statistical optimization of reaction parameters
A facile and efficient biogenic method was adopted to synthesize Bi2O3 and g-C3N4/Bi2O3 nanocomposites using Eichhornia crassipes plant extract. These composites were characterized by various analytical tools such as XRD, FTIR, SEM, TEM and UV-DRS. Bismuth oxide and g-C3N4/Bi2O3 show varied optical and photocatalytic properties due to the differences in their band gap. Here, Box-Behnken design (BBD) combined with Response Surface Methodology (RSM) has been used to optimize the coupled effect of independent parameters for the dye degradation. The synthesized g-C3N4/Bi2O3 nanocomposite exhibit excellent photocatalytic activity for the degradation of malachite green (MG) dye. Total Organic Carbon (TOC) analysis unveiled 78% photomineralization of MG over g-C3N4/Bi2O3 in 5 h. The p-n junction of g-C3N4/Bi2O3 with better oxidative ability and effective charge separation is the primary reason for its improved photocatalytic activity. The modified photocatalyst shows satisfactory catalytic activity and reusability towards photodegradation of common water pollutant MG up to 4 cycles. They offer great potential in the field of photocatalysis due to their superior efficiency and applications in environmental remediation. 2019 Elsevier B.V. -
Biogenic synthesis of g-C3N4/Bi2O3 heterojunction with enhanced photocatalytic activity and statistical optimization of reaction parameters /
Applied Surface Science, Vol.494, pp.465-476, ISSN No: 0169-4332. -
Biogenic synthesis of Pd-nanoparticles using Areca Nut Husk Extract: a greener approach to access ?-keto imides and stilbenes
An eco-friendly green method for a one-step synthesis of palladium nanoparticles and their synthetic utility are reported. Phytochemicals like amines, alcohols, and phenols present in the Areca Nut Husk extract facilitate the reduction of Pd(ii) to Pd(0). The phytochemicals serve as stabilising agents and ligands for palladium reduction and the need for an external ligand is avoided. The Field Emission Scanning Electron Microscopy and Transmission Electron Microscopy of newly synthesized palladium nanoparticles revealed a spherical morphology. The catalytic activity of the nanoparticles was tested for 1,2-difunctionalization of ynamides, Heck coupling, denitrogenative coupling of phenylhydrazine and C-H arylation of indole. Moreover, catalyst recyclability, control experiments, mechanistic elucidation, and gram-scale synthesis are elaborated. The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2021. -
Biogenic Synthesis of Zinc Oxide Nanoparticles Mediated by the Extract of Terminalia catappa Fruit Pericarp and Its Multifaceted Applications
Zinc oxide nanoparticles (ZnO-NPs) were biosynthesized by using the pericarp aqueous extract from Terminalia catappa Linn. These NPs were characterized using various analytical techniques such as X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, ultraviolet (UV) spectroscopy, dynamic light scattering (DLS), and scanning electron microscopy (SEM), and XRD studies of the nanoparticles reported mean size as 12.58 nm nanocrystals with highest purity. Further SEM analysis emphasized the nanoparticles to be spherical in shape. The functional groups responsible for capping and stabilizing the NPs were identified with FTIR studies. DLS studies of the synthesized NPs reported ? potential as ?10.1 mV and exhibited stable colloidal solution. These characterized ZnO-NPs were evaluated for various biological applications such as antibacterial, antifungal, antioxidant, genotoxic, biocompatibility, and larvicidal studies. To explore its multidimensional application in the field of medicine. NPs reported a potential antimicrobial activity at a concentration of 200 ?g/mL against bacterial strains in the decreasing order of Streptococcus pyogenes > Streptococcus aureus > Streptococcus typhi > Streptococcus aeruginosa and against the fungi Candida albicans. In vitro studies of RBC hemolysis with varying concentrations of NPs confirm their biocompatibility with IC50 value of 211.4 ?g/mL. The synthesized NPs DPPH free radical scavenging activity was examined to extend their antioxidant applications. The antiproliferation and genetic toxicity were studied with meristematic cells of Allium cepa reported with mitotic index (MI index) of 1.2% at the concentration of 1000 ?g/mL. NPs exhibited excellent Larvicidal activity against Culex quinquefasciatus larvae with the highest mortality rate as 98% at 4 mg/L. Our findings elicit the therapeutic potentials of the synthesized zinc oxide NPs. 2023 The Authors. Published by American Chemical Society -
Biogenic ZnO Nanoparticles Derived from Garcinia gummi-gutta Leaves: Synthesis, Characterization and its Multifaceted Applications
The current study focused on the bioreduction synthesis of ZnO nanoparticles using Garcinia gummi-gutta leaf extracts. The UV-vis analysis of the nanoparticles has reported the formation of an SPR peak at 379 nm. The functional groups taking part in the reduction reaction were analyzed using the FTIR technique and the average crystalline size of ZnO nanoparticles were found to be 22.27 nm from XRD measurements. The SEM and TEM images revealed the hexagonal shape of the nanoparticles with an average size 72.78 nm and 71.91 nm, respectively. Further, the synthesized nanoparticles were reported to be efficient degradation reactive textile dyes. The photodegradation results reported 92-100% degradation of the reactive dyes within 80-320 min. The antibacterial efficacy of the nanoparticles was investigated and the MIC of the nanoparticles was found to be 100 g/mL. The synthesized ZnO nanoparticles have exhibited significant cytotoxic effects on the MCF and HEP-G2 cell lines. 2024 Asian Publication Corporation. All rights reserved. -
Biological elimination of minerals from high ash coal by Aspergillus-like fungi
Efficiency of filamentous fungi such as Aspergillus niger on the bio-liquefaction of low rank Indian coals, its chemical composition, surface characteristics of the products and the microbial mechanisms of coal conversion were studied. Virgin and bio-liquefied/solubilized coal samples were characterized using FT-IR, Scanning electron microscopy and CHNS and proximate analysis. The micrographs were bright field and reveal several features correspond to the mineral grains comprising of aluminium, silicates and calcites. The absence of some morphological features corresponds to inorganic elements in residual samples which confirm demineralisation with the possible formation of respective Aluminum and Silicate complexes. The change in absorption of mineral matter functional group of these coal samples were studied using Fourier transform infra red spectroscopy (FT-IR). From the proximate analysis it was found that the ash content decreased by 76% when treated with fungal culture. Global Science Publications. -
Biological extraction of chitin from fish scale waste using proteolytic bacteria Stenotrophomonas koreensis and its possible application as an active packaging material
Chitin being the second most abundant polymer found in nature has extensive application and versatile material properties including biocompatibility. Extraction of chitin from diverse sources are majorly done using chemical extraction methods using high concentration of alkali that makes the method non eco-friendly and economically non-viable. This calls for eco-friendly methods of chitin extraction from cost-effective substrates through green methods. This research work presents a simplified one-step biological extraction of chitin from fish scales by successive fermentation using Stenotrophomonas koreensis isolated from soil. The fermentative approach for chitin extraction from fish scales using S. koreensis enzyme activity is not reported elsewhere in the available literature to the best of our knowledge. Chitin yield of 28% (w/w) was obtained after the successive fermentation. The extracted polymer was characterized using differential scanning calorimetry (DSC), Fourier transform infrared (FTIR), X-ray diffraction (XRD), and thermo gravimetric analysis (TGA). Furthermore, the possibility of converting extracted chitin into an active packaging material was explored by chemically, converting it to chitosan followed by analysis of its DPPH scavenging activity. The DPPH radical scavenging activity varied from 67.025 to 80.2%, which corresponds to 0.25 to 2mg/mL of chitosan. The chitosan films fabricated were subjected to biodegradation studies using soil burial method. Biodegradation rate of chitosan films was observed to be 21.49 0.62% (w/w) after 50days of incubation. Thus, the present research work highlights an integrated waste valorization strategy through microbial fermentation for commercially important biopolymer production. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. -
Biological Feature Selection and Classification Techniques for Intrusion Detection on BAT
Privacy is a significant problem in communications networks. As a factor, trustworthy knowledge sharing in computer networks is essential. Intrusion Detection Systems consist of security tools frequently used in communication networks to monitor, detect, and effectively respond to abnormal network activity. We integrate current technologies in this paper to develop an anomaly-based Intrusion Detection System. Machine Learning methods have progressively featured to enhance intelligent Anomaly Detection Systems capable of identifying new attacks. Thus, this evidence demonstrates a novel approach for intrusion detection introduced by training an artificial neural network with an optimized Bat algorithm. An essential task of an Intrusion Detection System is to maintain the highest quality and eliminate irrelevant characteristics from the attack. The recommended BAT algorithm is used to select the 41 best features to address this problem. Machine Learning based SVM classifier is used for identifying the False Detection Rate. The design is being verified using the KDD99 dataset benchmark. Our solution optimizes the standard SVM classifier. We attain optimal measures for abnormal behavior, including 97.2 %, the attack detection rate is 97.40 %, and a false-positive rate of 0.029 %. 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. -
Biomarker study of the biological parameter and neurotransmitter levels in autistics
Autism is a prevalent developmental disorder that combines repetitive behaviours, social deficits and language abnormalities. The present study aims to assess the autistic subjects using DSM IV-TR criteria followed with the analysis of neurotransmitters, biochemical parameters, oxidative stress and its ions in two groups of autistic subjects (group I < 12years; group II ? 12years). Antioxidants show a variation of 10% increase in controls compared to autistic age < 12years. The concentration of pyruvate kinase and hexokinase is elevated in controls approximately 60% and 45%, respectively, with the significance of 95 and 99%. Autistic subjects showed marked variation in levels of neurotransmitters, oxidative stress and its related ions. Cumulative assessment of parameters related to biochemical markers and neurotransmitters paves the way for autism-based research, although these observations draw interest in an integrated approach for autism. 2020, Springer Science+Business Media, LLC, part of Springer Nature. -
Biomass derived carbon quantum dots embedded PEDOT/CFP electrode for the electrochemical detection of phloroglucinol
Carbon nanocomposites have garnered a lot of attention among various nanomaterials due to their distinct characteristics, such as large surface area, biocompatibility, and concise synthetic routes. They are also a viable contender for electrochemical applications, notably sensing, due to their intriguing electrochemical features, which include large electroactive surface area, outstanding electrical conductivity, electrocatalytic activity, and high porosity and adsorption capability. Herein, an electrochemical sensor for phloroglucinol (PL) was designed using a CFP electrode modified with biomass-derived carbon quantum dots (S-CQD) doped on conducting organic polymer poly(3,4-ethylene dioxythiophene) (PEDOT) via electrodeposition method. The obtained nanocomposite (S-CQD+PEDOT) on the CFP electrode possesses a high surface area. The higher electrocatalytic activity of S-CQD and significant conductivity of PEDOT- modified electrode enhance the electrocatalytic activity for the phloroglucinol oxidation. The oxidation peak current of PL shows a higher response on the finally modified electrode than the other electrodes. The developed electrochemical sensor for the selective and sensitive detection of PL showed a good linear range of 36 -360 nM and a detection limit of 11 nM. The modified electrodes were characterized using Transmission electron spectroscopy (TEM), Fourier Transform infrared spectroscopy (FT-IR), and X-ray photon spectroscopy (XPS). Finally, the developed method was successfully used to detect Phloroglucinol from industrial effluents with RSD (0.841.02%) and (98.5101.2%) of recovery. 2023 -
Biomass-derived carbonaceous materials: Synthesis and photocatalytic applications /
Novel Applications of Carbon Based Nano-materials, 1st ed., pp.412-429, eBook ISBN : 9781003183549.