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Bioconversion of chicken feather waste into feather hydrolysate by multifaceted keratinolytic Bacillus tropicus LS27 and new insights into its antioxidant and plant growth-promoting properties
Abstract: Keratin, the main structural constituent of feathers, contains a lot of valuable amino acids which are potential bioactive compounds as well. Since conventional methods are not efficient enough to achieve complete removal of chicken feather waste, biological mode of feather degradation is one of the most appropriate ways to utilize feathers, thereby reducing wastes as well as generating value-added products from feathers. This study was focussed on valorizing chicken feather into feather hydrolysate (FH) containing bioactive compounds for plant growth promotion. Keratinolytic bacteria capable of degrading chicken feathers were isolated from the poultry waste dumping site of Russell Market, Shivajinagar, Bangalore, Karnataka, India. The isolated bacteria was identified as Bacillus tropicus LS 27. A minimal media with chicken feather as the sole source of carbon and nitrogen was prepared and inoculated with Bacillus tropicus LS 27 [5% (v/v)]. Degradation of keratin protein by bacteria caused the solubilization of amino acids which was confirmed by high-performance liquid chromatography (HPLC) analysis where an appreciable amount of amino acids like cysteine, valine, isoleucine, proline, lysine, methionine, and phenylalanine was detected. The Fourier transform infrared spectroscopy (FTIR) analysis of hydrolysed chicken feathers showed C=0 stretching, S-H bond stretching, and formation of carboxylic acid groups indicating effective degradation of chicken feathers. Scanning electron microscope (SEM) images revealed the degradation pattern of feathers showing complete degradation of barbs and barbules with a portion of rachis remaining. Feather hydrolysate was further explored for its antioxidant activity using DPPH scavenging assay, and the value was found to be 1.5 mg/mL. The bacterial cells when screened for heavy metal tolerance showed significant metal tolerance to lead (Pb) and chromium (Cr). Since Bacillus tropicus LS27 showed indole-3-acetic acid (IAA), siderophore, and ammonia production, the prepared feather hydrolysate along with the bacterial cells were used as soil amendment for plant growth studies over Spinacia oleracea L. The study revealed that plants supplemented with 20% (v/v) FH showed elevated plant growth, therefore proving to be optimum for the support of plant growth. Graphical abstract: [Figure not available: see fulltext.] 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. -
Bioconversion of chicken feather waste into feather hydrolysate by multifaceted keratinolytic Bacillus tropicus LS27 and new insights into its antioxidant and plant growth-promoting properties
Abstract: Keratin, the main structural constituent of feathers, contains a lot of valuable amino acids which are potential bioactive compounds as well. Since conventional methods are not efficient enough to achieve complete removal of chicken feather waste, biological mode of feather degradation is one of the most appropriate ways to utilize feathers, thereby reducing wastes as well as generating value-added products from feathers. This study was focussed on valorizing chicken feather into feather hydrolysate (FH) containing bioactive compounds for plant growth promotion. Keratinolytic bacteria capable of degrading chicken feathers were isolated from the poultry waste dumping site of Russell Market, Shivajinagar, Bangalore, Karnataka, India. The isolated bacteria was identified as Bacillus tropicus LS 27. A minimal media with chicken feather as the sole source of carbon and nitrogen was prepared and inoculated with Bacillus tropicus LS 27 [5% (v/v)]. Degradation of keratin protein by bacteria caused the solubilization of amino acids which was confirmed by high-performance liquid chromatography (HPLC) analysis where an appreciable amount of amino acids like cysteine, valine, isoleucine, proline, lysine, methionine, and phenylalanine was detected. The Fourier transform infrared spectroscopy (FTIR) analysis of hydrolysed chicken feathers showed C=0 stretching, S-H bond stretching, and formation of carboxylic acid groups indicating effective degradation of chicken feathers. Scanning electron microscope (SEM) images revealed the degradation pattern of feathers showing complete degradation of barbs and barbules with a portion of rachis remaining. Feather hydrolysate was further explored for its antioxidant activity using DPPH scavenging assay, and the value was found to be 1.5 mg/mL. The bacterial cells when screened for heavy metal tolerance showed significant metal tolerance to lead (Pb) and chromium (Cr). Since Bacillus tropicus LS27 showed indole-3-acetic acid (IAA), siderophore, and ammonia production, the prepared feather hydrolysate along with the bacterial cells were used as soil amendment for plant growth studies over Spinacia oleracea L. The study revealed that plants supplemented with 20% (v/v) FH showed elevated plant growth, therefore proving to be optimum for the support of plant growth. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. -
Bioconversion of Feather Composts using Proteolytic Bacillus mycoides for their Possible Application as Biofertilizer in Agriculture
Proteolytic Bacillus strains were screened for highest protease production amongst which Bacillus mycoides (G2) was chosen as an assuring protease producer. Enzyme activity was maximum at 37C, pH-7, when the medium was supplemented with 0.5 and 0.75% of sucrose and beef extract respectively. Tapioca flour and soybean meal were capable of replacing commercial carbon and nitrogen sources respectively. Feather degradation studies revealed 62% of degradation with Quail feather (QF), followed by Chicken feather (CF) (58%), Guinea fowl feather (51%) and Pigeon feather (43%). Biodegradation of feather samples in soil evidenced degradation of Quail feather and Chicken feather at the following patternQF Treatment 1 (5%) ? CF Treatment 1 (5%) ? QF Treatment 2 (10%) ? CF Treatment 2 (10%). Maximum degradation of QF and sufficient release of free amino acids into the feather compost was obvious with Field Emission Scanning Electron Microscopic (FE-SEM) and High Performance Thin Layer Chromatographic (HPTLC) analyses respectively. In vitro plant growth studies of tomato and chilly plants were accomplished with feather composts. Maximum growth of 26.44cm (shoot length) was achieved when feather compost prepared with degraded QF (5%) was utilized as plant growth substrate, than other treatment pots (P < 0.05). Plant growth was exemplary in the case of tomato when compared to that of chilly. Sound degradation of QF, followed by CF using Bacillus mycoides could strengthen the efficacy of microbial fermentation processes. This significant attempt could support poultry farms as well as organic agricultural sectors ecologically. Graphic Abstract: [Figure not available: see fulltext.] 2021, The Author(s), under exclusive licence to Springer Nature B.V. -
Biodegradable Organic Polymers for Environmental Protection and Remediation
As the era of urbanization and industrialization progressed, non-biodegradable polymers became a severe threat to the environment and the world's rapidly growing population. These synthetic polymers possess flexible applications and cost effectiveness which makes their usage more feasible and convenient. Today they are used from simple packaging to critical biomedical tools. Although these polymers possess many merits, all come to halt when it comes to biodegradability. The inherent mechanisms in nature are unable to degrade and decompose these synthetic polymers leading to their accumulation and persistence in nature for decades causing calamitous effects. In search of solutions for the adverse effects caused by synthetic polymers, the world turned toward biologically synthesized biodegradable organic polymers. These biopolymers have a diverse set of physical and chemical characteristics that can be easily manipulated, allowing for a wide range of applications. Biopolymers like polyhydroxyalkanoates and levan have adaptable qualities that resemble those of synthetic plastics which makes them a promising alternative to synthetic plastics. However, the setback in the large-scale usage of biopolymers is their high cost of production and commercialization. The biopolymers are broadly classified into three major classes based on their origin: plant-based biopolymers (cellulose, starch), animal-based biopolymers (chitin, chitosan, keratin), and microbial biopolymers (polyhydroxyalkanoates, levan). The extraction or synthesis of these biopolymers from their biological sources varies significantly from each other; however, in order to bring out the sustainable production, these polymers should be produced by coupling with waste valorization approaches. The waste materials from plants and animals, particularly agro-industrial wastes, can be used as inexpensive substrates for the commercial manufacture of these crucial biopolymers, thereby reducing the accumulation in the environment. Another field of biopolymer usage is in remediation of pollutants. Many biopolymers are currently being used in the active removal of heavy metal, dye, and other similar pollutants. The numerous physical, chemical, and biological processes for extraction or synthesis of industrially valuable biopolymers from the waste raw materials are discussed in this chapter, along with their application in remediation of pollutants and environmental protection. 2025 WILEY-VCH GmbH, Boschstra 12, 69469 Weinheim, Germany. All rights reserved. -
Biodegradation of polypropylene films by Bacillus paralicheniformis and Lysinibacillus fusiformis isolated from municipality solid waste contaminated soil
The fossil fuel or petroleum derived plastics are applied in our routine life because of their easy availability. Distribution and contamination of the plastics in the landfills are the major reasons for these biodegradation study. This current study reveals the biodegradation of polypropylene films and the growth of Bacillus paralicheniformis and Lysinibacillus fusiformis isolated from plastic contaminated soil collected from municipality solid waste management site. The degradation rate of PP films was confirmed by the results of biodegradation analysis. The growth of Bacillus paralicheniformis and Lysinibacillus fusiformis had shown OD values at 600nm after the degradation period of 4 weeks increasing from 0.131 to 0.334 and 0.148 to 0.213 respectively. The viable cell count increased from 804cells/ml to 1204cells/ml and 10.104cells/ml to 15.204cells/ml respectively. The physical and chemical changes of PP films were confirmed by FT-IR and XRD analysis. These analysis confirmed that the bacterial strains have the ability to change the chemical and physical nature of PP films and can utilize the PP films as sole carbon source. 2021 World Research Association. All rights reserved. -
Biodegradation studies of polyhydroxyalkanoates extracted from Bacillus subtilis NCDC 0671
The major characteristic feature that distinguishes polyhydroxyalkanoates (PHAs) from its synthetic counterparts is its biodegradability. PHAs are the only class of biopolymers reported to be 100% degradable under both aerobic and anaerobic conditions without production of any toxic residues. The biodegradability of PHAs is influenced by several factors like moisture, temperature, pH, surface area and molecular weight of the polymer. The rate of biodegradation varies greatly depending on the environment. Biodegradation studies were carried out using plating method and direct inoculation method using selected Bacillus strains. Fungal degradation of PHA sheets was assessed using Penicillium chrysogenum. Biodegradation of PHA sheets in different soil types like river valley, agricultural land and garden soil was investigated. The degree of PHA degradation in aqueous environment was studied by incubating the sheets in distilled water, sea water, fish tank water and pond water. The highest degradation rate was observed with agriculture land soil (35.47 0.13%) and fish tank soil (36.93 0.13%). The non-toxic nature of the soil incubated with PHA sheets was ensured using plant growth test. 2019, World Research Association. All rights reserved. -
Biodegradation studies of polyhydroxyalkanoates extracted from bacillus subtilis NCDC 0671 /
Research Journal of Chemistry And Environment, Vol.23, Issue 6, pp.107-114 -
Biodiversity and Indigenous Medicinal Knowledge of North-East India: Navigating Climate Change Impacts on Medicinal Plants for Conservation and Advancement
The northeastern region of India holds the sixth position among the world's 25 biodiversity hotspots, covering approximately 8% of the nation's total land area, which amounts to 262, 060 square kilometres. Situated in the eastern Himalayas, any alterations in this biodiversity-rich area can have significant and far-reaching consequences. Indigenous tribes of this region believe in the remarkable healing properties of certain medicinal plants, and within its diverse population of around 225 communities, each tribal and sub -tribal group possesses distinct traditional knowledge. Capturing and harnessing this indigenous wisdom by scientists and researchers could unlock new avenues for progress, particularly within the pharmaceutical sector. Climate change stands as one of the paramount global environmental challenges. Predictions indicate that by the close of the 21st century, the Earth's average temperature might rise by anywhere between 0.3 to 4.8 C, accompanied by a potential sea level increase of 26 to 82 cm. These climate shifts could have adverse effects on the abundance and accessibility of medicinal plants, potentially leading to species extinction. Moreover, the impact of climate change could extend beyond availability to also encompass alterations in the pharmacological properties of various plants, particularly those found in alpine environments. This discussion underscores the importance of existing knowledge, critical analyses, challenges, opportunities, and the immense value of medicinal plants. It emphasizes the intersection of changing climate and the vulnerability of medicinal plant resources, necessitating a comprehensive understanding of these effects in the context of the North Eastern region of India. To address these challenges, there is a pressing need for in-depth research on the geographical distribution of plant communities and strategies to enhance the secondary synthesis of critically endangered medicinal plants under the current climate change scenarios. The Author(s). -
Bioengineering of biowaste to recover bioproducts and bioenergy: A circular economy approach towards sustainable zero-waste environment
The inevitable need for waste valorisation and management has revolutionized the way in which the waste is visualised as a potential biorefinery for various product development rather than offensive trash. Biowaste has emerged as a potential feedstock to produce several value-added products. Bioenergy generation is one of the potential applications originating from the valorisation of biowaste. Bioenergy production requires analysis and optimization of various parameters such as biowaste composition and conversion potential to develop innovative and sustainable technologies for most effective utilization of biowaste with enhanced bioenergy production. In this context, feedstocks, such as food, agriculture, beverage, and municipal solid waste act as promising resources to produce renewable energy. Similarly, the concept of microbial fuel cells employing biowaste has clearly gained research focus in the past few decades. Despite of these potential benefits, the area of bioenergy generation still is in infancy and requires more interdisciplinary research to be sustainable alternatives. This review is aimed at analysing the bioconversion potential of biowaste to renewable energy. The possibility of valorising underutilized biowaste substrates is elaborately presented. In addition, the application and efficiency of microbial fuel cells in utilizing biowaste are described in detail taking into consideration of its great scope. Furthermore, the review addresses the significance bioreactor development for energy production along with major challenges and future prospects in bioenergy production. Based on this review it can be concluded that bioenergy production utilizing biowaste can clearly open new avenues in the field of waste valorisation and energy research. Systematic and strategic developments considering the techno economic feasibilities of this excellent energy generation process will make them a true sustainable alternative for conventional energy sources. 2023 Elsevier Ltd -
Biofabricated textiles The future of sustainable fashion
[No abstract available] -
Biofortification and Sustainable Intensification of Soil: Perspectives on Rice Cultivation in India
Biofortified crops have inherently been known to acquire climate-smart traits and stress resilience. Climate-smart agriculture integrates climate change into the planning and implementation of sustainable agricultural strategies. Biofortification is a climate-smart concept that enhances crop nutrient quality and quantity through conventional breeding, agronomic practices, or genetic engineering. It will enrich food availability, stability, accessibility, and utilization and positively impact the health, livelihood, production, and distribution of food crops. The system of rice (Oryza sativa L.) intensification involves a set of agronomic principles to improve the structure and functioning of the soil system by fortifying it with organic matter and micronutrients. With the exceeding urbanization and population explosion, food security is a primary concern for policymakers all around the globe. Widespread zinc, iodine, iron, and selenium micronutrient malnutrition is a significant cause of numerous health problems in human populations where rice is part of the staple diet. Climate-smart biofortification is a durable and effective option to reach the vast numbers of malnourished populations scattered across the world sustainably. Approaches have been strategized worldwide under rice biofortification research projects for maintaining, increasing, and introducing new micronutrients in rice grain. Biofortification has been safely implemented as an environmentally friendly approach to produce higher yields at low costs without undesirable soil effects. Prospective advancements can be achieved by integrating mineral and organic fertilizers with superior germplasm, promoting improved nutrient uptake and localization in the consumed parts of the crop. 2025 The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG. -
Biofuel production and characterization from waste chicken skin and pig fat
The biofuels are the most important alternative energy sources in future to fulfil the energy demands. The team of our students carried out an innovative process to convert waste to value-added products. The students have been visited many meat stalls and gathered the required amount of resources with and without cost. The collected waste chicken skin and pig tallow is heated and extracted fat, which is the primary sources to produce the biofuel. The fat extraction process was carried by shredding down the waste chicken skin and pig tallow. The obtained fat was filtered and heated up to 110C to remove all the impurities, water suspensions, blood cells and pieces of bones. The process called transesterification process was carried out to convert obtained fat into biofuel with methyl alcohol and KOH as a catalyst. Transesterification process carted with fat before acid wash and after acid wash to examine the effect of FFA on biofuel yield. The quantity of biofuel yield has been observed to be 62 to 68% for fat from waste chicken skin and 82 to 83 % for fat from pig tallow. The derived fuel from fat from both resources is combined with conventional diesel fuel to check the different properties on a volume basis varied by 10% up to 40%. The essential properties such as viscosity, density, flashpoint, fire point and calorific values were determined, and results show that the fuel combination CB20 and PB20 meets the all requirements of ASTM standards to fix as an additive fuel to CI engines. The clear biofuel from both the fat expressed higher viscosity, density, flash and fire point with a lesser value of energy density. BEIESP. -
Biofuel production and characterization from waste chicken skin and pig fat /
International Journal of Recent Technology And Engineering, Vol.8, Issue 3, pp.3598-3603, ISSN No: 2277-3878. -
Biofuels from bio-waste and biomass
The planet's limited natural fossil fuel reserves are anticipated to be very soon owing to massive usage. Biofuels would be a critical alternative source that may reduce global warming and CO2 emissions. The food-versus-fuel dilemma is, however, one of the key drawbacks of first-generation biofuels like corn ethanol, sugarcane ethanol, etc. Cellulose and hemicellulose, the primary constituents of lignocellulosic feedstocks, could be reduced to sugars by either thermochemical/biological processes before being fermented to generate biofuels. However, owing to structural heterogeneity, more complicated operational techniques are required before the production technology can be commercialized, and several challenges must be addressed. This chapter provided an assessment of various feedstocks, availability, various processing techniques, obstacles, and current technical developments in the generation of biofuels from biomass. 2023, IGI Global. -
Biofunctional NiTiO3D-carvone nanocomposite: Synthesis, characterization, antibacterial, antioxidant, and zebrafish biocompatibility evaluation
The growing threat of microbial resistance and oxidative stress-related disorders highly demands the development of multifunctional nanoparticles with enhanced bioactivity and biocompatibility. In response to this demand, D-carvone functionalized nickel titanate (NiTiO?-D-carvone) was synthesized. XRD confirmed the formation of a crystalline rhombohedral NiTiO3 phase, while HRTEM revealed reduced agglomeration and uniform distribution upon D-carvone modification. The average crystallite size of the composite was 27 nm. DLS analysis showed a decreased hydrodynamic diameter (D?? = 112.50 nm) for the functionalized nanocomposite compared to bare NiTiO3 (225.70 nm). XPS spectra confirmed the presence of Ni? and Ti?? oxidation states, along with C=O and CO groups from D-carvone. The NiTiO3D-carvone nanocomposite exhibited a surface area of 2.658 m/g and showed strong UV absorption at 326 nm. PL studies revealed that the nanocomposite exhibited green emission at 524 nm, attributed to oxygen vacancy-related defect states which is favorable for enhanced ROS generation. Antibacterial studies demonstrated enhanced inhibition zones against K. pneumoniae (16 mm), S. dysenteriae (17 mm), Escherichia coli (18 mm) P. aeruginosa (12 mm) and P. vulgaris (17 mm) compared to bare NiTiO?. CFU assays revealed a concentration-dependent reduction in viable colonies, with the highest activity at 10 mg/mL. The antioxidant efficiency reached 61.4 % DPPH inhibition at 20 g/mL, closely comparable to Vitamin C (64.7 %). Zebrafish embryo assays confirmed excellent biocompatibility of the NiTiO?D-carvone system, with normal morphology and survival up to 96 hpf. The experimental results suggest that surface modification with D-carvone significantly enhances the biological activity and safety of NiTiO3 nanoparticles. 2025 -
Biofunctional TiVO4chitosanL-histidine hybrid nanomaterials for enhanced antimicrobial and anticancer applications
The growing pervasiveness of multidrug-resistant (MDR) pathogens and the limitations of conventional chemotherapeutics demand the development of multifunctional nanomaterials with enhanced efficacy and biocompatibility. In this study, titanium vanadate (TiVO4) nanoparticles and TiVO4chitosanL-histidine (TiVO4CsLH) HNM's were successfully synthesized via a wet chemical solgel route followed by surface functionalization. Structural analysis confirmed the formation of phase-pure tetragonal TiVO4 with an average crystallite size of ?38 nm, which was significantly reduced to ?24 nm upon CsLH functionalization. UVVisible spectroscopy revealed band gap narrowing from 4.75 eV (TiVO4) to 4.15 eV (TiVO4CsLH), indicating modified electronic structure and improved light absorption. The TiVO4CsLH HNM's exhibited superior broad-spectrum antimicrobial activity, with inhibition zones ranging from 18 to 19 mm against Gram positive, Gram negative and fungal strain, outperforming TiVO4. In vitro anticancer evaluation against MCF-7 breast cancer cells demonstrated pronounced concentration- and time-dependent cytotoxicity, with IC50 values decreasing from 29.8 ?g mL?1 (24 h) to 20.6 ?g mL?1 (72 h), significantly lower than those of TiVO4. Biocompatibility studies using L929 fibroblast cells revealed high cell viability (>82%) even at 60 ?g mL?1, which confirms the selective anticancer activity of TiVO4-Cs-LH HNMs. The enhanced biological performance of the TiVO4CsLH HNM's arose from synergistic effects of reduced crystallite size, improved dispersion, defect-assisted charge separation, and biofunctional surface chemistry, making it a promising candidate for antimicrobial and anticancer applications. 2026 Elsevier B.V. -
Biogenesis and Green Synthesis of Metal Nanoparticles and Their Pharmacological Applications
Nanomaterial innovation is the primary catalyst of advancement in nanotechnology. Although there are many known chemical processes for creating nanoparticles that use harmful substances, it is now more important than ever to use processes that are safer, greener, and more environmentally friendly. The goal of research in this field is to use diverse life forms as "nanoparticle factories." Phytochemicals can convert salt into the appropriate nanoparticles thanks to their regular biosynthetic routes. In recent years, green chemistry methods for the synthesis of metallic nanoparticles have emerged as a fresh and exciting area of study. Metal nanoparticles, including gold (Au), silver (Ag), iron (Fe), and cadmium (Cd) along with certain oxides, can be synthesized using a variety of chemical and physical techniques as well as biological techniques carried out using plants. It has been discovered that methods involving plant-mediated synthesis are a more efficient and cost-effective way to create these metal nanoparticles. The plant-mediated nanoparticles are used as potential pharmaceutical agents for many diseases, including hepatitis, cancer, malaria, and HIV. Due to the higher efficacy and fewer side effects of nanodrugs compared to other commercial cancer drugs, the synthesis of nanoparticles targeting biological pathways has gained tremendous popularity. This review paper aims to cover the different green methods for the biogenesis of these nanoparticles, the different compounds and salts used, and the metals obtained. Ultimately, the significance and prospects of these metal nanoparticles especially in the fields of medicine, pharmacology, drug designing, and drug delivery engineering will also be commented on. The Author(s). -
Biogenic Carboxymethyl Cellulose and L-Histidine Coated Barium Peroxide Nanocomposite: A Tri-Functional Platform Targeting Antimicrobial Resistance, Cancer, and Oxidative Stress
The study addresses the urgent need for novel therapeutic agents in combating multidrug-resistant pathogens, cancer, and oxidative stress-related diseases. Barium peroxide (BaO2) nanoparticles and its novel nanocomposite, carboxymethyl cellulose and L-histidine coated BaO2 (BaO2-CMC-LH) were synthesized, characterized, and evaluated for their antibacterial, anticancer, and DPPH radical scavenging activities. Structural and functional characterizations were carried out using various analytical techniques. Biological evaluations demonstrated enhanced antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) and Candida albicans (C. albicans), with BaO2-CMC-LH exhibiting superior inhibition zones compared to BaO2. For MRSA, the zone of inhibition (ZOI) for BaO2 is about 9 mm, whereas the BaO2-CMC-LH nanocomposite exhibits a substantially larger ZOI of 21 mm. Likewise, for C. albicans, BaO2 presents a ZOI of 12 mm, and BaO2-CMC-LH exhibits a greater inhibition zone of 18 mm. Cytotoxicity analysis against osteosarcoma MG-63 cells revealed significantly improved anticancer activity, with BaO2-CMC-LH achieving an IC?? value of 28.6 ?g/mL compared to 53.3 ?g/mL for BaO2. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay showed that the BaO2-CMC-LH nanocomposite (78.45%) had activity compared to BaO2 alone (64.43%). These findings underscore the synergistic effects of the CMC-LH matrix in enhancing BaO2's biological activities, positioning BaO2-CMC-LH as a promising multifunctional therapeutic agent for addressing global health challenges. 2026 John Wiley & Sons Ltd. -
Biogenic Copper Oxide@rGO Nanocomposite for Decontamination of a Food Threat B. Cereus in a Rice Model
Pathogenic microorganisms have become a serious threat to human beings all over the world as they cause severe disease illnesses. This study used supercritical carbon dioxide as a green solvent to prepare a nanocomposite composed of copper oxide (CuO) nanoparticles dispersed on the surface of reduced graphene oxide (rGO). The resulting nanocomposite was examined using a various of alanytical techniques. The developed CuO/rGO nanocomposite exhibited potenail antimicrobial ability against a food pathogenic bacterium, Bacillus cereus. Additionality, it showed high toxicity towards B. cereus, confirmed by the means of fluorescent live-dead counting of cells. Its mechanistic role against a food meneac was confirmed by the means of time-kill ability (complete inhibition at 200min), cell membrane integrity (OD of control: 1.62; CuO/rGO: 3.78 at 60min), membrane uptake (Control: 27.21%; Cuo/rGO: 64.33%), and membrane potential ability (Control: 58.33; CuO/rGO: 24.12) towards B. cereus. Study of scanning electron microscopy analyse resulted in the membrane disruption of B. cereus by the nanocomposite. Moreover, the CuO/rGO nanocomposite inhibited in vitro biofilm formation ability (Crystal violet uptake - Control: 31.33%; CuO/rGO: 59.11%) of B. cereus. Furthermore, the nanocomposite coating was used as a rinse solution for rice bowl packages. Interestingly, a rinsing solution (20% and 4060min) significantly inhibited the CFU count of B. cereus in rice by 2.1 log cfu/g as compaered to control 6.2 log cfu/g. The outcomes highlight the effectiveness of nanocomposite coating against food menace B. cereus, suggesting that the developed nanocomposite could be applied as an effective antimicrobial marinade and/or a rinse for raw rice preservation aginst hazardous foodborne pathogens. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2026. -
Biogenic synthesis of dopamine/carboxymethyl cellulose/TiO2 nanoparticles using Psidium guajavaleaf extract with enhanced antimicrobial and anticancer activities
The green synthesis of metal oxide nanoparticles (NPs) has garnered considerable attention from researchers due to its utilization of eco-friendly solvents during synthesis and cost-effective approaches. This study focuses on the synthesis of titanium oxide (TiO2) and dopamine (DA) carboxymethyl cellulose (CMC)-doped TiO2 (DA/CMC/TiO2) NP using Psidium guajava leaf extract, while also investigating the structural, optical, and morphological and biocidal potential of the prepared NPs. Significantly larger zones of inhibition were observed for DA/CMC/TiO2 NPs compared to TiO2 against various pathogens. Moreover, the MTT assay was carried out to evaluate the anticancer activity of the prepared samples against MG-63 cells, and the results revealed that DA/CMC/TiO2 NPs exhibited significantly higher level of anticancer activity compared to TiO2. The experimental results demonstrated that DA/CMC/TiO2 NPs exhibited enhanced anticancer activity in a dose-dependent manner when compared to TiO2 NPs. 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.